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  2. Mouse embryonic fibroblast - Wikipedia

    en.wikipedia.org/wiki/Mouse_embryonic_fibroblast

    A photo of human embryonic stem cells (the cell colonies in the center). Spindle cells surrounding the stem cell colony are MEFs. Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line.

  3. 3T3 cells - Wikipedia

    en.wikipedia.org/wiki/3T3_cells

    The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the second "3") continuously. [2] The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3 ...

  4. Cell fate determination - Wikipedia

    en.wikipedia.org/wiki/Cell_fate_determination

    The developmental equivalence of these cells is usually established via transplantation and cell ablation experiments. As embryos mature, more complex fate determination occurs as structures appear, and cells differentiate, beginning to perform specific functions.

  5. Fibroblast - Wikipedia

    en.wikipedia.org/wiki/Fibroblast

    In view of the potential clinical applications of stem cell-derived tissues or primary epithelial cells, the use of human fibroblasts as an alternative to MEF feeders has been studied. [14] Whereas the fibroblasts are usually used to maintain pluripotency of the stem cells, they can also be used to facilitate development of the stem cells into ...

  6. Embryoid body - Wikipedia

    en.wikipedia.org/wiki/Embryoid_body

    EBs are differentiation of human embryonic stem cells into embryoid bodies comprising the three embryonic germ layers. They mimic the characteristics seen in early-stage embryos. They are often used as a model system to conduct research on various aspects of developmental biology.

  7. Myogenesis - Wikipedia

    en.wikipedia.org/wiki/Myogenesis

    Myogenesis is the formation of skeletal muscular tissue, particularly during embryonic development. Muscle fibers generally form through the fusion of precursor myoblasts into multinucleated fibers called myotubes. In the early development of an embryo, myoblasts can either proliferate, or differentiate into a myotube. What controls this choice ...

  8. Germline development - Wikipedia

    en.wikipedia.org/wiki/Germline_development

    In the mouse, primordial germ cells (PGCs) arise in the posterior primitive streak of the embryo [4] and start to migrate around 6.25 days after conception. PGCs start to migrate to the embryonic endoderm and then to the hindgut and finally towards the future genital ridges where the somatic gonadal precursors reside.

  9. Germ layer - Wikipedia

    en.wikipedia.org/wiki/Germ_layer

    The ectoderm generates the outer layer of the embryo, and it forms from the embryo's epiblast. [13] The ectoderm develops into the surface ectoderm, neural crest, and the neural tube. [14] The surface ectoderm develops into: epidermis, hair, nails, lens of the eye, sebaceous glands, cornea, tooth enamel, the epithelium of the mouth and nose.