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Water for injection is generally made by distillation or reverse osmosis. [5] It should contain less than a mg of elements other than water per 100 ml. [5] Versions with agents that stop bacterial growth are also available. [5] In the UK, some hospitals offer subcutaneous injections of water directly for treating back pain in labour.
In this method a measured sub-sample (perhaps 10 ml) is diluted with 100 ml of sterile growth medium and an aliquot of 10 ml is then decanted into each of ten tubes. The remaining 10 ml is then diluted again and the process repeated. At the end of 5 dilutions this produces 50 tubes covering the dilution range of 1:10 through to 1:10000.
A bacteriostatic agent or bacteriostat, abbreviated Bstatic, is a biological or chemical agent that stops bacteria from reproducing, while not necessarily killing them otherwise. Depending on their application, bacteriostatic antibiotics , disinfectants , antiseptics and preservatives can be distinguished.
Benzyl alcohol has moderate solubility in water (4 g/100 mL) and is miscible in alcohols and diethyl ether. The anion produced by deprotonation of the alcohol group is known as benzylate or benzyloxide .
It is recommended for use in cell culture applications at a concentration of 10 ml per liter. It is the most common antibiotic solution for the culture of mammalian cells and it does not have any adverse effects on the cells themselves. [2] It was first introduced in 1955 in cell culture. [3]
Bacteriostatic agents have been effectively used for treatment that are considered to require bactericidal activity. Furthermore, some broad classes of antibacterial agents considered bacteriostatic can exhibit bactericidal activity against some bacteria on the basis of in vitro determination of MBC/MIC values.
Completely de-gassed ultrapure water has a conductivity of 1.2 × 10 −4 S/m, whereas on equilibration to the atmosphere it is 7.5 × 10 −5 S/m due to dissolved CO 2 in it. [10] The highest grades of ultrapure water should not be stored in glass or plastic containers because these container materials leach (release) contaminants at very low ...
[9] [10] MIC values provide a quantitative measure of an extract or compound's antimicrobial potency. The lower the MIC, the more potent the antimicrobial. [4] When in vitro toxicity data is available, MICs can also be used to calculate selectivity index values, a measure of off-target to target toxicity. [4]
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