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Lignin is found to be degraded by enzyme lignin peroxidases produced by some fungi like Phanerochaete chrysosporium. The mechanism by which lignin peroxidase (LiP) interacts with the lignin polymer involves veratrole alcohol , which is a secondary metabolite of white rot fungi that acts as a cofactor for the enzyme.
Lignin-modifying enzymes benefit industry as they can break down lignin; a common waste product of the paper and pulp industry. These enzymes have been used in the refinement of poplar as lignin inhibits the enzymatic hydrolysis of treated poplar and Lignin-modifying enzymes can efficiently degrade the lignin thus fixing this problem. [4 ...
Production of lignin-peroxidase and manganese-peroxidase is the hallmark of basidiomycetes and is often used to assess basidiomycete activity, especially in biotechnology applications. [38] Most white-rot species also produce laccase, a copper-containing enzyme that degrades polymeric lignin and humic substances. [39]
Aside from wine, laccases are of interest in the food industry, [21] [22] [23] including food packaging. [24] The ability of laccases to modify complex organic molecules has attracted attention in the area of organic synthesis. [25] Laccases have been also been studied as catalysts in bioremediation to degrade emerging pollutants and ...
The conventional method for lignin quantitation in the pulp industry is the Klason lignin and acid-soluble lignin test, which is standardized procedures. The cellulose is digested thermally in the presence of acid. The residue is termed Klason lignin. Acid-soluble lignin (ASL) is quantified by the intensity of its Ultraviolet spectroscopy.
The black liquor is an aqueous suspension of lignin residues, hemicellulose, and the inorganic chemicals used in the process. The black liquor comprises 15% solids by weight of which two thirds are organic chemicals and the remainder are inorganic. [3] Normally the organics in black liquor are 40-45% soaps, [4] 35-45% lignin and 10-15% other ...
Ester linkages arise between oxidized sugars, the uronic acids, and the phenols and phenylpropanols functionalities of the lignin. To extract the fermentable sugars, one must first disconnect the celluloses from the lignin, and then use acid or enzymatic methods to hydrolyze the newly freed celluloses to break them down into simple monosaccharides.
Lignin, comprising a major fraction of biomass, is sometimes classified according to the guaiacyl component. Pyrolysis of lignin from gymnosperms gives more guaiacol, resulting from removal of the propenyl group of coniferyl alcohol. These lignins are said to have a high guaiacyl (or G) content.