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Structure of a major component of the dye nigrosin. In staining dyes, nigrosin (CI 50415, Solvent black 5) is a mixture of black synthetic dyes made by heating a mixture of nitrobenzene, aniline, and hydrochloric acid in the presence of copper or iron. [1] Related to induline, it is a mixture of phenazine-based compounds.
For bright-field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin and India ink.The specimen, such as a wet bacterial culture spread on a glass slide, is mixed with the negative stain and allowed to dry.
Basic red 5 50040 eurhodin 553-24-2: New fuchsine: Magenta III Basic violet 2 42520 triarylmethane 3248-91-7: New methylene blue: NMB Basic blue 24 52030 thiazine 1934-16-3: Night blue Basic blue 15 44085 triarylmethane 4692-38-0: Nigrosin: Solvent black 5 50415 azine 11099-03-9: Nigrosin WS Acid black 2 50420 azine 8005-03-6: Nile blue: Nile ...
Adjective dyes are those dyes that require use of a mordant to bind the color to the fiber. [2] alkanet Alkanet or dyer's bugloss (Alkanna tinctoria) is a traditional plant source of red dye. [3] alum Alum (aluminum sulfate) is a naturally occurring basic mordant widely used in the ancient world. [4] añil
Unlike negative staining, positive staining uses basic dyes to color the specimen against a bright background. While chromophore is used for both negative and positive staining alike, the type of chromophore used in this technique is a positively charged ion instead of a negative one.
Many aminophenazines are prominent dyes. Two of the first synthetic dyes are aminophenazines, these include induline and nigrosin. [2] The symmetrical diaminophenazine is the parent substance of the important dyestuff neutral red (dimethyldiaminotoluphenazine). It is obtained by the oxidation of ortho-phenylenediamine with ferric chloride.
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Basic copper carbonate: Cu 2 (OH) 2 CO 3. Iron pigments. Prussian blue (PB27): a synthetic inert pigment made of iron and cyanide: C 18 Fe 7 N 18. Manganese pigments. YInMn Blue: a synthetic pigment discovered in 2009 (YIn 1−x Mn x O 3). [4] Manganese blue: barium manganate(VI) sulfate.