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The following equation is used to calculate the number of colony forming units (CFU) per ml from the original aliquot / sample: CFU per ml = Average number of colonies for a dilution x 50 x dilution factor. Advantages. Faster than other methods. Produce less bacterial contamination of the working surface.
From 2008, Field Ornithology Group conducts a bird count in December in the lines of Christmas Bird Count by the National Audubon Society of the United States. [5] Sri Lanka's location at the tip of the Indian subcontinent has made the island a termination point in the North-South bird migratory route. [6]
The "dilution factor" is an expression which describes the ratio of the aliquot volume to the final volume. Dilution factor is a notation often used in commercial assays. For example, in solution with a 1/5 dilution factor (which may be abbreviated as x5 dilution ), entails combining 1 unit volume of solute (the material to be diluted) with ...
A dilution of the cells to be counted is prepared and mixed with Trypan blue, this is normally the stain of choice because it is taken up by dead cells and actively excluded from live cells. Once the cells have been stained, they are counted using a hemocytometer, then a calculation is carried out to the original concentration of live cells. [1]
The garden includes more than 4000 species of plants, including orchids, spices, medicinal plants and palm trees. [3] Attached to it is the " National Herbarium of Sri Lanka ". The total area of the botanical garden is 147 acres (0.59 km 2 ), at 460 meters above sea level, and with a 200-day annual rainfall.
One of the most important features of chemostats is that microorganisms can be grown in a physiological steady state under constant environmental conditions. In this steady state, growth occurs at a constant specific growth rate and all culture parameters remain constant (culture volume, dissolved oxygen concentration, nutrient and product concentrations, pH, cell density, etc.).
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For example, if you plate 1x 10 7 cells and count 1000 colonies, the transformation efficiency is: (1000/1x 10 7) x 100 = 0.1% Alternatively, CFUs can be reported per microgram of DNA used for the transformation. This can be calculated by multiplying the number of colonies by the volume of the culture plated and dividing by the amount of DNA used.