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Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).
Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same phase. Cell synchrony is a vital process in the study of cells progressing through the cell cycle as it allows population-wide data to be collected rather than relying solely on single-cell experiments.
Propidium iodide is used as a DNA stain in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, [2] or in microscopy to visualize the nucleus and other DNA-containing organelles. Propidium Iodide is not membrane-permeable, making it useful to differentiate necrotic, apoptotic and healthy cells based on membrane ...
An embedded graph uniquely defines cyclic orders of edges incident to the same vertex. The set of all these cyclic orders is called a rotation system.Embeddings with the same rotation system are considered to be equivalent and the corresponding equivalence class of embeddings is called combinatorial embedding (as opposed to the term topological embedding, which refers to the previous ...
The eukaryotic cell cycle is very complex and is one of the most studied topics, since its misregulation leads to cancers. It is possibly a good example of a mathematical model as it deals with simple calculus but gives valid results. Two research groups [1] [2] have produced several models of the cell cycle simulating several organisms. They ...
In eukaryotes, the cell cycle consists of four main stages: G 1, during which a cell is metabolically active and continuously grows; S phase, during which DNA replication takes place; G 2, during which cell growth continues and the cell synthesizes various proteins in preparation for division; and the M phase, during which the duplicated ...
Computers are critical to analysis and modelling of these data. The goal is to create accurate real-time models of a system's response to environmental and internal stimuli, such as a model of a cancer cell in order to find weaknesses in its signalling pathways, or modelling of ion channel mutations to see effects on cardiomyocytes and in turn ...
The flow across the cells is determined based on μ(k) and λ(k), two monotonic functions that uniquely define the fundamental diagram as shown in Figure 1. The density of the cells is updated based on the conservation of inflows and outflows. Thus, the flow and density are derived as: Where: and represent density and flow in cell i at time t.