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Ion-exchange resin beads Ion-exchange column used for protein purification. Ion exchange is a reversible interchange of one species of ion present in an insoluble solid with another of like charge present in a solution surrounding the solid. Ion exchange is used in softening or demineralizing of water, purification of chemicals, and separation ...
Ion-exchange resin beads. An ion-exchange resin or ion-exchange polymer is a resin or polymer that acts as a medium for ion exchange, that is also known as an ionex. [1] It is an insoluble matrix (or support structure) normally in the form of small (0.25–1.43 mm radius) microbeads, usually white or yellowish, fabricated from an organic polymer substrate.
Resins (often termed 'beads') of ion exchange columns may include functional groups such as weak/strong acids and weak/strong bases. There are also special columns that have resins with amphoteric functional groups that can exchange both cations and anions. [ 33 ]
Schematic structure of DEAE-C: positively charged diethylaminoethanol groups can bind negative ions. Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion-exchange chromatography, a type of column chromatography, for the separation and purification of proteins and nucleic acids.
For anionic resins, regeneration typically uses a solution of sodium hydroxide or potassium hydroxide. The waste waters eluted from the ion-exchange column containing the unwanted calcium and magnesium salts are typically discharged to the sewage system. [3] Recharge typically takes the following steps: [10]
Anion-exchange chromatography is a process that separates substances based on their charges using an ion-exchange resin containing positively charged groups, such as diethyl-aminoethyl groups (DEAE). [2] In solution, the resin is coated with positively charged counter-ions . Anion exchange resins will bind to negatively charged molecules ...
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