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The aim of an accurate intraocular lens power calculation is to provide an intraocular lens (IOL) that fits the specific needs and desires of the individual patient. The development of better instrumentation for measuring the eye's axial length (AL) and the use of more precise mathematical formulas to perform the appropriate calculations have significantly improved the accuracy with which the ...
In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens. Without oil, light waves reflect off the slide specimen ...
Viscosity index. The viscosity index (VI) is an arbitrary, unit-less measure of a fluid's change in viscosity relative to temperature change. It is mostly used to characterize the viscosity-temperature behavior of lubricating oils. The lower the VI, the more the viscosity is affected by changes in temperature.
Typical oils used have an index of refraction around 1.515. [2] An oil immersion objective is an objective lens specially designed to be used in this way. The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion.
The Becke line test is a technique in optical mineralogy that helps determine the relative refractive index of two materials. It is done by lowering the stage (increasing the focal distance) of the petrographic microscope and observing which direction the light appears to move. This movement will always go into the material of higher refractive ...
In optics, the refractive index (or refraction index) of an optical medium is a dimensionless number that gives the indication of the light bending ability of that medium. Refraction of a light ray. The refractive index determines how much the path of light is bent, or refracted, when entering a material. This is described by Snell's law of ...
Total internal reflection fluorescence microscope. A total internal reflection fluorescence microscope (TIRFM) is a type of microscope with which a thin region of a specimen, usually less than 200 nanometers can be observed. TIRFM is an imaging modality which uses the excitation of fluorescent cells in a thin optical specimen section that is ...
A refractometer is a laboratory or field device for the measurement of an index of refraction (refractometry). The index of refraction is calculated from the observed refraction angle using Snell's law. For mixtures, the index of refraction then allows the concentration to be determined using mixing rules such as the Gladstone–Dale relation ...