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An autoclave is a machine used to carry out industrial and scientific processes requiring elevated temperature and pressure in relation to ambient pressure and/or temperature. Autoclaves are used before surgical procedures to perform sterilization and in the chemical industry to cure coatings and vulcanize rubber and for hydrothermal synthesis ...
Using mice as test animals, one experiment showed that heating BSE positive brain tissue at 134–138 °C (273–280 °F) for 18 minutes resulted in only a 2.5 log decrease in prion infectivity. [15] Most autoclaves have meters and charts that record or display information, particularly temperature and pressure as a function of time.
Moist heat sterilization processes sterilize using hot air that is heavily laden with water vapor, which plays the most important role in the sterilization. [ 1 ] [ 2 ] Boiling a sample for 30 minutes or more will kill virtually all vegetative cells present, but will not kill spores , which can germinate shortly thereafter and resume growth.
where g is the number of degrees below the retort temperature on a simple heating curve at the end of the heating period, B B is the time in minutes from the beginning of the process to the end of the heating period, and f h is the time in minutes required for the straight-line portion of the heating curve plotted semilogarithmically on paper ...
This open system allows researchers to maintain the exponential growth phase of cells for use in physiological experiments. [ 1 ] A chemostat (from chem ical environment is stat ic) is a bioreactor to which fresh medium is continuously added, while culture liquid containing left over nutrients, metabolic end products and microorganisms is ...
In microbiology, in the context of a sterilization procedure, the D-value or decimal reduction time (or decimal reduction dose) is the time (or dose of an antimicrobial drug) required, at a given condition (e.g. temperature) or set of conditions, to achieve a one-log reduction, that is, to kill 90% of relevant microorganisms. [1]
A complete cycle involves heating the oven to the required temperature, maintaining that temperature for the proper time interval for that temperature, turning the machine off and cooling the articles in the closed oven till they reach room temperature. The standard settings for a hot air oven are: 1.5 to 2 hours at 160 °C (320 °F)
Biofluid Dynamics refers to the study of fluid Dynamics of basic biological fluids such as blood, air etc. and has immense applications in the field of diagnosing, treating and certain surgical procedures related to the disorders/diseases which originate in the body relating to cardiovascular, pulmonary, synovial systems etc.