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The catalase test is done by placing a drop of hydrogen peroxide on a microscope slide. An applicator stick is touched to the colony, and the tip is then smeared onto the hydrogen peroxide drop. If the mixture produces bubbles or froth, the organism is said to be 'catalase-positive'. Staphylococci [47] and Micrococci [48] are
A fibrin clot formed in a test tube by the coagulase reaction. The tube test uses rabbit plasma that has been inoculated with a staphylococcal colony (i.e., Gram-positive cocci which are catalase positive). The tube is then incubated at 37 °C for 1.5 hours. If negative, then incubation is continued up to 18 hours.
The catalase test tests whether a microbe produces the enzyme catalase, which catalyzes the breakdown of hydrogen peroxide. Smearing a colony sample onto a glass slide and adding a solution of hydrogen peroxide (3% H 2 O 2) will indicate whether the enzyme is present or not. Bubbling is a positive test while nothing happening is a negative ...
The main criterion for differentiation between Staphylococcus spp. and Streptococcus spp. is the catalase test. Staphylococci are catalase positive whereas streptococci are catalase-negative. [2] S. pyogenes can be cultured on fresh blood agar plates.
The aerotolerance of C. tertium can lead to its misidentification as Bacillus spp. or Lactobacillus spp. [2] A negative catalase test is an easy tool to differentiate C. tertium from Bacillus spp., which are catalase positive. [2] Also, C. tertium only forms spores anaerobically, as opposed to Bacillus spp., which sporulates aerobically. [2]
It is a catalase-positive, [10] coagulase-negative, facultative anaerobe that can grow by aerobic respiration or by fermentation. Some strains may not ferment. [3] [11] Biochemical tests indicate this microorganism also carries out a weakly positive reaction to the nitrate reductase test.
A. faecalis is a Gram-negative bacterium which appears rod-shaped and motile under a microscope. It is positive by the oxidase test and catalase test, but negative by the nitrate reductase test. It is alpha-hemolytic and requires oxygen. A. faecalis can be grown at 37 °C, and forms colonies that lack pigmentation. [1]
Staphylococci spp. are a genus of gram positive cocci of 0.5 - 1 μm diameter. [citation needed] Staphylococcus pseudintermedius is a non-motile and non-spore forming, facultatively anaerobic bacterium. [citation needed] It appears primarily as grape-like clusters morphologically, but can also be seen as individual or paired cocci.